Journal: Journal for Immunotherapy of Cancer

Article Title: Inhibition of stromal MAOA leading activation of WNT5A enhance prostate cancer immunotherapy by involving the transition of cancer-associated fibroblasts

doi: 10.1136/jitc-2024-010555

Figure Lengend Snippet: Suppressing stromal MAOA activity in vivo can effectively inhibit the proliferation of prostate cancer xenografts by potentiating the immune-killing function of CD8 + T cells. ( A ) Schematic diagram of the construction of a subcutaneous tumor graft model in C57BL/6J mice and the drug administration strategy. ( B ) Tumor growth curves of mice in different treatment groups. ( C ) Representative images and quantification results of KI-67 + and Tunel + in mice from different treatment groups, scale bars, 50 µm. ( D ) Representative images and quantification results of CD8 + T cells in different treatment groups of mice, scale bars, 50 µm. ( E ) FACS analysis of the proportion of CD8 + CD45 + T cells in tumor tissues from different treatment groups. ( F ) ELISA experiments to detect the content of IFN-γ in tumor tissues from different treatment groups. ( G ) Strategy for the construction of double humanized PCa mouse model and administration. ( H ) The tumor growth curves of mice in the NC group and the sh-MAOA group. ( I ) Representative images and quantification results of KI-67 + and Tunnel + for mice in the NC group and sh-MAOA group, scale bars, 50 µm. ( J ) Representative images and quantification results of CD8 + T cells in the NC group and sh-MAOA group of mice. Scale bars, 50 µm. ( K ) FACS analysis of the proportion of CD8 + CD45 + T cells in tumor tissues from NC group and sh-MAOA group. ( L ) ELISA experiments to detect the content of IFN-γ in tumor tissues from NC group and sh-MAOA group. Clo, chlorgyline; Con, control; DAPI, 4',6-Diamidino-2-Phenylindole; FACS, fluorescence-activated cell sorting; M-PrSC, mouse primary stromal cells; NC, negative control; PBMC, peripheral blood mononuclear cell; PCa, prostate cancer; Phe, phenelzine; sh-MAOA, short hairpin RNA targeting monoamine oxidase A.

Article Snippet: Human PCa cell lines (PC3, DU145, C42, 22RV1), mouse PCa cell lines (RM-1, Tramp-C1), Human prostate stromal myofibroblast (WPMY-1) and Human leukemia cell line (JURKAT) were obtained from the American Type Culture Collection (Manassas, Virginia, USA) and cultured in Roswell Park Memorial Institute 1640 and Dulbecco’s Modified Eagle’s Medium supplemented with 10% fetal bovine serum (Gibco).

Techniques: Activity Assay, In Vivo, TUNEL Assay, Enzyme-linked Immunosorbent Assay, Control, Fluorescence, FACS, Negative Control, shRNA

Journal: Journal for Immunotherapy of Cancer

Article Title: Inhibition of stromal MAOA leading activation of WNT5A enhance prostate cancer immunotherapy by involving the transition of cancer-associated fibroblasts

doi: 10.1136/jitc-2024-010555

Figure Lengend Snippet: Inhibition of stromal MAOA sensitizes prostate cancer cells to the therapeutic effects of immune checkpoint inhibitors. ( A ) Strategy for the construction of subcutaneous tumor xenograft model in C57BL/6J mouse model and administration. ( B ) Tumor growth curves of mice in different treatment groups. ( C ) The expression of WNT5A, the proportion of CD8 + T cells, and the content of IFN-γ in different treatment groups of mice tumor tissues. ( D ) Strategy for the construction of double humanized PCa mouse model and administration. ( E ) Tumor growth curves of mice in different treatment groups. ( F ) The expression of WNT5A, the proportion of CD8 + T cells, and the content of IFN-γ in different treatment groups of mice tumor tissues. ( G ) A schematic diagram of the mechanism by which inhibiting MAOA in tumor-associated fibroblasts exerts antitumor effects. Availability of data and material: the datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request. CAF, cancer-associated fibroblast; Clo, chlorgyline; Con, Control; MAOA, monoamine oxidase A; M-PrSC, mouse primary stromal cells; ns, not significant; PBMC, peripheral blood mononuclear cell; PCa, prostate cancer; PD-1, programmed cell death protein-1; ROS, Reactive Oxygen Species; sh-MAOA, short hairpin RNA targeting MAOA; WT, Wild Type.

Article Snippet: Human PCa cell lines (PC3, DU145, C42, 22RV1), mouse PCa cell lines (RM-1, Tramp-C1), Human prostate stromal myofibroblast (WPMY-1) and Human leukemia cell line (JURKAT) were obtained from the American Type Culture Collection (Manassas, Virginia, USA) and cultured in Roswell Park Memorial Institute 1640 and Dulbecco’s Modified Eagle’s Medium supplemented with 10% fetal bovine serum (Gibco).

Techniques: Inhibition, Expressing, Generated, Control, shRNA